rat il1β probe  (Advanced Cell Diagnostics Inc)

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 1. Representative fluorescent micrographs of whole-mount preparations of myenteric ganglia from the duodenum, ileum and colon of control, diabetic, and insulin-treated diabetic rats after IL1β-HuC/HuD double-labelling immunohistochemistry. HuC/HuD as a pan-neuronal marker was applied to label myenteric neurons. CD—control duodenum (a), CI—control ileum (b), CC—control colon (c), DD—diabetic duodenum (d), DI—diabetic ileum (e), DC—diabetic colon (f), IDD—insulin- treated diabetic duodenum (g), IDI—insulin-treated diabetic ileum (h), IDC—insulin-treated diabetic colon (i); arrows—IL1β-immunoreactive myenteric neurons, arrowheads—myenteric neurons. Scale bars: 20 µm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Control, Immunohistochemistry, Marker

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 2. Proportion of IL1β-immunoreactive myenteric neurons in the duodenum, ileum, and colon of control rats. The proportion of IL1β-immunoreactive myenteric neurons was significantly higher in the colon compared to the ileum and duodenum. Data are expressed as mean ± SEM. **** p < 0.0001 (relative to control duodenum); oooo p < 0.0001 (between control ileum and colon).

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Control

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 3. Proportion of IL1β-immunoreactive myenteric neurons of the duodenum, ileum, and colon of control, diabetic and insulin-treated diabetic rats. In the diabetics, the proportion of IL1β- immunoreactive myenteric neurons was significantly increased in all gut segments, which was prevented by immediate insulin treatment. Data are expressed as mean ± SEM. ** p < 0.01 (relative to controls); o p < 0.05, oooo p < 0.0001 (between diabetics and insulin-treated diabetics). C—controls, D—diabetics, ID—insulin-treated diabetics.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Control

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 4. Representative fluorescent micrographs of whole-mount preparations of myenteric ganglia from the colon of control (a), diabetic (b), and insulin-treated diabetic (c) rats after IL1β-nNOS double-labelling immunohistochemistry. CC—control colon, DC—diabetic colon, IDC—insulin- treated diabetic colon; arrows—IL1β-nNOS-immunoreactive myenteric neurons, arrowheads—nNOS- immunoreactive neurons. Scale bars: 20 µm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Control, Immunohistochemistry

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 5. Proportion of IL1β-nNOS-immunoreactive neurons related to the total number of nNOS- immunoreactive neurons in the myenteric ganglia of the duodenum, ileum, and colon of control, diabetic and insulin-treated diabetic rats. The proportion of IL1β-nNOS-immunoreactive neurons significantly increased only in the colon of diabetics relative to controls. Data are expressed as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 (relative to controls); oo p < 0.01 (between diabetics and insulin-treated diabetics). C—controls, D—diabetics, ID—insulin-treated diabetics.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Control

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 6. Representative fluorescent micrographs of whole-mount preparations of myenteric ganglia from the ileum of control (a) and diabetic (b) rats after IL1β-CGRP double-labelling immunohisto- chemistry. CI—control ileum, DI—diabetic ileum; arrows—IL1β-CGRP-immunoreactive myenteric neurons, arrowheads—CGRP-immunoreactive neurons. Scale bars: 20 µm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Control, Immunohistochemistry

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 9. Representative micrographs of cryosections of myenteric ganglia from the colon of control (a), diabetic (b) and insulin-treated diabetic (c) rats after IL1β RNAscope. IL1β mRNA appear as green punctate dots (arrows), nuclei were counterstained with DAPI (blue). CC—control colon, DC—diabetic colon, IDC—insulin-treated diabetic colon. Scale bars: 20 µm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Control, RNAscope

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 10. Representative micrographs of cryosections of mucosa from the duodenum of control (a), diabetic (b) and insulin-treated diabetic (c) rats after IL1β RNAscope. IL1β mRNA appear as green punctate dots (arrows), nuclei were counterstained with DAPI (blue). CD—control duodenum, DD—diabetic duodenum, IDD—insulin-treated diabetic duodenum. Scale bars: 20 µm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Control, RNAscope

Journal: International journal of molecular sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats.

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Figure 11. Expression of IL1β mRNA in the myenteric ganglia (a), smooth muscle (b), and intestinal mucosa (c) of the duodenum, ileum, and colon of control, diabetic and insulin-treated diabetic rats. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001 (relative to controls); o p < 0.05, oo p < 0.01 (between diabetics and insulin-treated diabetics). C—controls, D—diabetics, ID—insulin-treated diabetics.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 ◦C.

Techniques: Expressing, Control